ap endonuclease, ref-1, ape1/ref-1, apex1, ape/ref-1, apurinic/apyrimidinic endonuclease 1, ap lyase, ape-1, endo iii, ap-endonuclease, more top print hide show all columns Go to Synonym Search Please wait a moment until the data is sorted.

In biochemistry, a lyase is an enzyme that catalyzes the breaking (an "elimination" reaction) of various chemical bonds by means other than hydrolysis (a "substitution" reaction) and oxidation, often forming a new double bond or a new ring structure.

Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 150 µL purified antibody (0.17 mg/mL). Adenylosuccinate lyase antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No.

Ap lyase

NEIL3 has AP lyase activity on ssDNA. Glycosylitic release of oxidized bases by the Fpg‐Nei glycosylase family is followed by the β–δ elimination reaction of the associated AP lyase, resulting in … 2010-04-11 Excision of AP sites near DSBs by a 5'dRP/AP lyase is thus critical for efficient and accurate resolution of such ends by cellular NHEJ (Figure 1a). Consistent with other studies 14 , 15 (reviewed in 16 ) an alternative to NHEJ (Alt-NHEJ) allows for significant joining of undamaged ends in Ku70 deficient cells ( Figure 1d , columns 1 and 2, 7 and 8). DNA-(apurinic or apyrimidinic site) lyase.

Complete information for OGG1 gene (Protein Coding), 8-Oxoguanine DNA Glycosylase, including: function, proteins, disorders, pathways, orthologs, and expression. AP synonyms, AP pronunciation, AP translation, English dictionary definition of AP. abbr. 1.

Function i Bifunctional DNA glycosylase/lyase, which excises 5-methylcytosine (5-meC) and 5-hydroxymethylcytosine (5-hmeC), leaving an apyrimidinic (AP) site that is subsequently incised by the lyase activity (PubMed: 25240767).

In Arabidopsis, AtOGG1 is a DNA glycosylase/apurinic/apyrimidinic (AP) lyase that is involved in base excision repair for eliminating 8-oxo-G from DNA. In this study, the functions of AtOGG1 were elaborated. 8-oxo-dG glycosylase/AP lyase activity in crude mitochondrial extracts was measured by an oligonucleotide incision assay, as previously described . Briefly, RHM or RLM were resuspended in a buffer containing 20 mM HEPES-KOH (pH 7.6), 1 mM EDTA, 2 mM DTT, 300 mM KCl, 5% glycerol and 0.05% Triton X-100. The AP-lyase activity cleaves both 3´ and 5´ to the AP site thereby removing the AP site and leaving a 1 base gap.

The AP-lyase activity cleaves both 3´ and 5´ to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B 1 -fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil (1,2).

American plan 4. antipersonnel 5. Associated Press 6. author's proof American Heritage® Dictionary of the We conducted a biochemical characterization of the AP lyase activities of Ntg1p and Ntg2p via a series of kinetic experiments. Such studies were designed to determine if Ntg1p and Ntg2p prefer speci®c bases located opposite abasic sites and whether these lesions are processed with a catalytic ef®ciency similar to Apn1p, the major hydrolytic AP endo-nuclease of yeast. ATP citrate lyase (ACLY) Gene ontology (GO) analysis was performed with probes with a p < 0.05 and a >1.5‐fold change relative to the control siRNA.

In reactions reconstituted with uracil-DNA glycosylase (UDG), apurinic/apyrimidinic (AP) endonuclease and DNA ligase I, pol ι can use its dRP lyase and polymerase Gamma Cystathionase antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, mouse, rat samples. Cat.No. 12217-1-AP. KD/KO Validated.
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AP Endonucleases. AP lyase.

AP-endonuclease klyver 5'-end, Lyase A 3-hydroxy-3-methylglutaryl-CoA lyase gene in the .. Surrender at 20: Urf Post Collection: URF ending on April A 3-hydroxy-3-methylglutaryl-CoA lyase gene The identification of ATP-citrate lyase as a protein kinase B (Akt) substrate in Duncan, G.; Webb, S. F.; Dawson, A. P.; Bootman, M. D. and Elliott, A. J. (1993). Themmen AP, de Jong Fh, Fauser BCJM .
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N -glycosylase activity releases damaged purines, including 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 8-oxo-7,8-dihydroguanine (8oxoG), generating an AP site. The AP lyase activity cleaves an AP site, via β and δ-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini. See more details on Bioz

Also cleaves the DNA backbone at apurinic/apyrimidinic sites (AP sites) (PubMed:10521423, PubMed:19446526). Has little specificity for the base opposite oxoG (PubMed:10521423). 2010-12-21 (redirected from AP lyase) APEX1 A gene on chromosome 14q11.2 that encodes a major apurinic/apyrimidinic (AP) endoDNAase, which is involved in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents, … 2012-04-02 During AP site cleavage by the AP lyase activity of DNA glycosylases, an aldimine intermediate between the C1′ atom of deoxyribose and a primary amine group in the protein is formed The AP-lyase activity cleaves both 3´ and 5´ to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B 1 -fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil (1,2). The AP lyase experiments with different Topo-V fragments suggested that the lyase active site is located between residues 556 and 599. The fact that there is no AP lyase activity using a THF-containing substrate suggested that Topo-V is similar to other DNA repair proteins that use a Lys residue for nucleophilic attack on the DNA backbone (17,27). NEIL3 has AP lyase activity on ssDNA.

A new class of nucleic acid substrates for AP endonucleases and members of the glycosylase/lyase family of enzymes is described. Representatives of each family, the enzymes Nfo and fpg, respectively, cleave nucleic acid backbones at positions in which a base has been replaced by a linker to which a variety of label moieties may be attached.

Such studies were designed to determine if Ntg1p and Ntg2p prefer speci®c bases located opposite abasic sites and whether these lesions are processed with a catalytic ef®ciency similar to Apn1p, the major hydrolytic AP endo-nuclease of yeast. Proteintech Anti-ATP citrate lyase Polyclonal, Catalog # 15421-1-AP.

Segen's Medical Dictionary. © 2012 Farlex, Inc. The studies described here extend the biochemical characterization of two of these enzymatic activities using human ALKBH1: the AP lyase and 6-methyl adenine DNA demethylase activities. The apurinic/apyrimidinic (AP) site is considered to be a common lesion in genomic DNA, arising at a frequency of 10,000 to 50,000 lesions per mammalian cell per day (1).